A Generalized Design for Affinity Chromatography Columns
نویسندگان
چکیده
منابع مشابه
Design and recognition properties of a hydropathically complementary peptide to human interleukin 1 beta.
A computer-designed hydropathically complementary peptide to human interleukin 1 beta (IL1 beta) precursor sequence 204-215 recognized the 204-215 peptide as well the entire IL1 beta protein with binding affinities in the micromolar range. Interaction between the complementary pair was characterized by analytical high-performance liquid affinity chromatography on columns derivatized with the co...
متن کاملLectin purification on affinity columns containing reductively aminated disaccharides.
A method is described for isolating lectins in pure form and quantitative yield in a single step by affinity chromatography on aminoethyl polyacrylamide gels containing reductively aminated disaccharide residues. The affinity columns were prepared in two steps: (a) direct reductive amination of the disaccharide and aminoethyl gel with sodium cyanoborohydride in aqueous solution at pH 9; (b) N-a...
متن کاملCriteria for affinity chromatography of steroid-binding macromolecules.
When soluble fractions of rat kidney homogenates were exposed to affinity chromatography columns containing deoxycorticosterone covalently linked to an insoluble agarose matrix, aldosterone-binding activity was reduced by 92%. Similarly, when renal homogenates were exposed to affinity chromatography columns containing estriol linked to agarose, renal estradiol-binding activity was reduced by 93...
متن کاملCentrifugal system for affinity chromatography with eluate monitoring.
A prototype centrifugal system has been developed that permits parallel photometric monitoring of eluate streams from a radial array of chromatographic columns. The modular rotor design consists of a discoidal center insert for eluent and sample apportionment, the chromatographic columns, and flow-through cuvetts-all mounted on an aluminum base plate. A common sample is introduced simultaneousl...
متن کاملDevelopment of HPLC methods for the purification and analysis of plasma membrane glycoproteins.
High resolution HPLC techniques such as affinity chromatography (AC), ion exchange chromatography (IEC), and size exclusion chromatography (SEC) were used successfully for separations of hydrophobic plasma membrane glycoproteins. We have tested a lot of commercially available columns for IEC and SEC and performed the purification of the crude plasma membrane extract with the most suitable colum...
متن کامل